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Concise

Concise Separations specializes in the synthesis of polymeric beads, latexes, linear polymers, as well as functionalization of beads in unique ways for manufacture of HPLC columns in the Biofuels, Food and Beverage, Water Analysis, and Pharmaceutical Manufacturing markets. Concise Separations is uniquely qualified to develop customized, high-quality columns for whatever a user may need.

  • Carbohydrate Columns are used for analysis of monosaccharaides, disaccharides, oligosaccharides, sugar alcohols, syrups, various beverages, pharmaceuticals, biomass, and biofuels.

  • Organic Acid Columns are used for analysis of monosaccharaides, disaccharides, alcohols, fermentation, pharmaceuticals, and biofuel.

  • Anion Columns are used for drinking water, waster water, corrosion analysis, pollution control, and medicinal applications. 

  • Amino Acid Columns are used for protein hydrolysates and complicated physiological assays.

  • Nucleic Acid Columns are used for ssDNA, dsDNA, mRNA, rRNA, siRNA, and oligonucleotides.


Carbohydrate Columns

Concise Separations offers carbohydrate columns with different metal ligands to best suit your separation needs. Lower cross linkage allows for improved oligosaccharide separation while a higher degree of cross linkage allows for faster flow rates. Additionally, we offer a variety of bead and column sizes, so we can make the best column to optimize your carbohydrate assays.

  • Calcium Columns are used for routine analysis of syrups, mono- and disaccharides with and without organic acids.

      Why Calcium?

    • Ion Exclusion columns with the Calcium ligand provide a high degree of selectivity and are easier to clean than a Lead ligand column.

    • Calcium Carbohydrate Columns are the work horse for mono- and disaccharide analysis.

  • Lead Columns are used for complicated analysis, unknowns, and bio-mass/biofuel analysis.

      Why Lead?

    • Ion Exclusion columns with the Lead ligand provide the highest degree of selectivity needed for unknowns and complicated samples.

    • The Lead moiety allows for a stronger interaction with sugars which drastically improves analyte separation.

  • Sodium Columns are used for oligosaccharide analysis and separations in a brine environment.

      Why Sodium?

    • Ion Exclusion columns with the Sodium ligand provides a high degree of selectivity in a moderate to high level of salinity.

    • The Sodium moiety allows for a strong interaction with sugar, sugar alcohols, and oligosaccharides and can be used with PAD systems.

  • Potassium Columns are used for sugar beet analysis when the separation of the zwitterion, Betaine, is critical.

      Why Potassium?

    • Ion Exclusion columns with the Potassium ligand provide a higher surface affinity for complex sugars

    • Excellent selectivity for syrup and molasses analysis.


Organic Acid Columns

Concise Separations Coregel series of Ion Exclusion columns were made for easy use and fast analysis of organic acids, sugar alcohols, aldehydes, carbohydrates and alcohols. We have created columns with various cross linkages and configurations to produce the best separation for each user’s needs. Thus, we offer the customized column for Fermentations, Biomass, Biofuels, Beverages, Pharmaceutical and R&D. We offer different bead and column sizes, so we can make the best column to optimize your organic acid assays.

  • 6% Cross-Linked Columns are used for unknowns and complicated samples.

      Why 6% Cross Linkage?

    • A 6% cross linked Ion Exclusion column, in the hydrogen form, has the highest degree of selectivity for organic acids and carbohydrates.
  • 7% Cross-Linked Columns are used for unknowns and bio fuel analysis for sugar, organic acid, and alcohols.

      Why 7% Cross Linkage?

    • A 7% cross linked Ion Exclusion column, in the hydrogen form, has a very high degree of selectivity for organic acids and alcohols.

    • Operate with more ruggedness than the 6% columns.

  • 8% Cross-Linked Columns are used for routine analysis of fermentation products, bio fuels, and beverages.

      Why 8% Cross Linkage?

    • A 8% cross linked Ion Exclusion column, in the hydrogen form, is a very rugged bead that has a high degree of selectivity for organic acids, alcohols, as well as mono and disaccharides.

  • 10% Cross-Linked Columns are used for routine analysis of fermentation products, bio fuels, and beverages.

    • Why 10% Cross Linkage?

    • A 10% cross linked Ion Exclusion column, in the hydrogen form, is a very rugged bead that still has a high degree of selectivity for organic acids, aldehydes, and alcohols.

    • The extra cross linkage allows for faster flow rates than the 8% or 6% cross linked columns.


Anion Columns

The ICSep Series of Ion Chromatography columns gives our customers improved tools to optimize water analysis. Concise Separations offers columns for very fast analysis to high capacity columns for better control on elution times. Additionally we offer different bead and column sizes, so you can choose what sizes are optimal for your anion assays.

Unique Guard Disc™ technology incorporated in most ICSep columns give added protection from metals and organic contaminants. Suppressed, non-suppressed, solvent compatibility and soda analysis are all features that Concise Separations has mastered since the first anion exchange column made in the late 1980s.

  • High Capacity Columns are needed for samples containing Oxyhalides (EPA Drinking Water Method 300.b) or to isolate a large concentration of one anion from target anions.

      Why High Capacity?

    • They are solvent compatible and can be cleaned and ran with MeOH, MeCN etc.

    • Simple carbonate/bicarbonate eluent is easy to prepare and suppress.

  • Fast Elution Columns are used because the Drinking Water Standard for U.S. EPA Drinking Water Method 300.a calls for a quick run time for the 7 Standard Anions.

      Why Fast Elution?

    • Columns on this page are designed to be ran at 2 mL/min to meet EPA standards.

    • Simple carbonate/bicarbonate eluent is easy to prepare and suppress.

  • Non-Suppressed Columns are used to eliminate the need for complicated suppressor equipment, waste, and expensive system replacement.

      Why Non-Suppressed?

    • A simple buffered salicylic eluent is used for these columns that are designed for non-suppressed anion chromatography.

    • The ICSep AN1 column can be used for both suppressed and non-suppressed conductivity detection.

  • Speciality IC Columns include our AN SDA column and our CN3 column.

      Why AN SDA?

    • Designed to separate anions in untreated cola and other soda beverages.

    • Caramel coloring will not affect the column lifetime or chromatography and the AN SDA is solvent compatible.

      Why CN3?

    • Designed for divalent cation analysis.

    • Excellent selectivity with fast elution times.

  • AN1 columns are designed for suppressed conductivity detection.

      Why AN1?

    • Unique selectivity pulls fluoride away from water dip while organic acids resolved and divalent anions quickly eluted.

    • Simple carbonate/bicarbonate eluent is easy to prepare and suppress.


    Amino Acid Columns

    Concise Separations AminoSep series of Amino Acid columns are designed for easy use and high selectivity. Simple eluents combined with temperature and post column reactionsproduces dialed-in separations needed for the demands of Protein Hydrolysates and the most complicated Physiological samples.

    Renowned for polymer lot to lot, column to column reproducibility and consistency. Additionally, we offer different bead and column sizes, so you can choose what sizes are optimal for your amino acid assays. Concise Separations set the bar in Amino Acid Separation and has made the amino acid columns sold by Beckman for decades.

    Why Sodium?

    • High degree of selectivity and robustness that is need for complicated Protein Hydrolysate assays.

    • Nanogram sensitivity when used with OPA post column reaction.

    Why Lithium?

    • Highest degree of selectivity that is needed for complicated Physiological assays.

    • Have the highest efficiencies and resolution. 

    • Designed for post column reaction with Ninhydrin.


    Nucleic Acid Columns

    Concise Separations has been manufacturing HPLC columns for nucleic acid separations since we helped invent Denaturing HPLC back in 1994. When these columns are used in ion-pairing mode, they are ideal for purification of ssDNA, dsDNA,mRNA, rRNA, siRNA and oligonucleotides from 10 bases to 100k bases.  If you need pure fragments, then Concise Separations has the nucleic acid column for you.

    Why DNASep?

    • For over two decades the DNASep column line has been the leader in sensitivity and separation power for DNA fragments. 

    • Can easily detect mutations in under 5 minutes

    Why RiboSep RNA?

    • A single column that can separate siRNA as well as large mRNA with a simple change ingradient. 

    • Use the analytical 50 x 4.6mm format for precision separations and to identify contaminants. 

    • Use the SemiPrep 100 x 21.2mm format for purification. 

    • Both formats offer no sample carry-over and high resolution.

    Why OligoSep?
    • We offer both non-porous (precision separations) and porous media (higher capacity needed inpurification assays). 

    • Long lasting columns yield high resolution in oligonucleotide separations. 

    • We offer different formats and can custom make a column to best fit your application.

    Why Reverse Phase RPSep?

    • Perfect for biological samples that have an acidic or basic matrix. 

    • Polymeric columns are pH stable from 0 to 14. 

    • No need for shielding as these columns are robust due to highly cross-linked polystyrene polymer. 

    • You can even use a column oven to enhance separations as the PRX-1 polymeric beads do not decompose.

    Why ProteinSep?

    • Powerful polymeric reversed phase column. 

    • The 2um packing gives high resolution and the non-porous surface eliminates sample carry over.